Abstract:
Abstract: Agro wastes such as carrot peel, onion peel, potato peel and sugar beet peel were
utilized for the production of cellulase. The agro wastes produced cellulase enzyme and
activity was obtained on 6th day for potato peel and 7th day for carrot peel, onion peel and
sugar beet peel. The maximum cellulase activity was observed with sugar beet for all
parameters; at the pH of 6.5 (1.68 U/ml) and at temperature 40ºC (1.76U/ml). With different
carbon and nitrogen sources, the maximum enzyme activity was observed with manitol and
sugar beet peel as substrate (2.32U/ml) and with potassium nitrate, sugar beet peel also as
substrate (1.69 U/ml). The kinetics enzyme has optimal temperature of 40°C, optimal pH of
8, the ions of Mg2+, Mn2+ and cu2+ and EDTA in low concentrations of 2mM. EDTA was
found to inhibit enzyme ctivity of cellulase, while the Zn2+ and Fe2+ activated the cellulase.
Different metal ions increased ordecreased inhibition of cellulase activity at 2mM. Michaelis-
Menten equation constant (Km and Vmax) values of purified cellulase were 0.22 μg /mL and
2.34 U/ml.
