Agro wastes residues as strategy to produce cellulase.

Abstract

Abstract: Agro wastes such as carrot peel, onion peel, potato peel and sugar beet peel were utilized for the production of cellulase. The agro wastes produced cellulase enzyme and activity was obtained on 6th day for potato peel and 7th day for carrot peel, onion peel and sugar beet peel. The maximum cellulase activity was observed with sugar beet for all parameters; at the pH of 6.5 (1.68 U/ml) and at temperature 40ºC (1.76U/ml). With different carbon and nitrogen sources, the maximum enzyme activity was observed with manitol and sugar beet peel as substrate (2.32U/ml) and with potassium nitrate, sugar beet peel also as substrate (1.69 U/ml). The kinetics enzyme has optimal temperature of 40°C, optimal pH of 8, the ions of Mg2+, Mn2+ and cu2+ and EDTA in low concentrations of 2mM. EDTA was found to inhibit enzyme ctivity of cellulase, while the Zn2+ and Fe2+ activated the cellulase. Different metal ions increased ordecreased inhibition of cellulase activity at 2mM. Michaelis- Menten equation constant (Km and Vmax) values of purified cellulase were 0.22 μg /mL and 2.34 U/ml.